患儿男，3岁。因发热半天，惊厥1次于春季人院。査体：T390C，昏睡状，前囟膨隆，张力高，颈抗阳性，双下肢见散在瘀点、瘀斑。心肺腹无异常，颈阻阳性，双侧克氏征、布氏征阴性。以下哪项检查最有助于明确诊断

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