患儿2岁半，病初2天有轻微咳嗽，随后出现高热，体温达400C,烦躁，频繁呕吐，査体：神志清楚，颈项强直，脑膜刺激征阳性，巴宾斯基征阳性；脑脊液检查外观混浊，压力增高，白细胞计数2000x106/L,以多核细胞为主，糖2.2mmol/L，蛋白质1100mg/L。应先采取的进一步检查是

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