以H1N1病毒核衣壳蛋白为抗原，采用细胞工程制备单克隆抗体，下列叙述正确的是

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• 1下列关于植物体细胞杂交的叙述，错误的是

  A.不同种植物原生质体融合的过程属于植物体细胞杂交过程

  B.原生质体仍具有再生细胞壁，连续分裂并长成完整植株的能力

  C.参与杂种细胞的细胞壁形成的细胞器主要是线粒体和核糖体

  D.植物体细胞杂交技术可克服不同种植物之间的不亲和障碍

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• 2下列有关植物体细胞杂交技术的描述，不正确的是

  A.利用植物体细胞杂交可以获得某种多倍体植株

  B.获得原生质体的过程中需要纤维素酶和果胶酶

  C.通常用聚乙二醇或灭活病毒诱导原生质体融合

  D.可根据细胞中染色体数目和形态的差异来鉴定杂种细胞

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• 3进行生物工程设计时，下表各组所选择的实验材料与实验目的配置错误的是  <table style="BORDER-COLLAPSE: collapse; MARGIN-LEFT: 10.6pt; mso-table-layout-alt: fixed; mso-padding-alt: 0cm 0cm 0cm 0cm" border="0" cellspacing="0" cellpadding="0" _dj="MsoNormalTable"><tbody><tr style="HEIGHT: 15.5pt; mso-yfti-irow: 0; mso-yfti-firstrow: yes"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1.5pt solid; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 29.55pt; PADDING-RIGHT: 0cm; HEIGHT: 15.5pt; BORDER-TOP: windowtext 1.5pt solid; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm" width="39">组别</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 128.5pt; PADDING-RIGHT: 0cm; HEIGHT: 15.5pt; BORDER-TOP: windowtext 1.5pt solid; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt" width="171">实验目的</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 100.75pt; PADDING-RIGHT: 0cm; HEIGHT: 15.5pt; BORDER-TOP: windowtext 1.5pt solid; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt" width="134">实验材料</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 156.8pt; PADDING-RIGHT: 0cm; HEIGHT: 15.5pt; BORDER-TOP: windowtext 1.5pt solid; BORDER-RIGHT: windowtext 1.5pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt" width="209">材料特点</td></tr><tr style="HEIGHT: 16.6pt; mso-yfti-irow: 1"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1.5pt solid; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 29.55pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-top-alt: solid windowtext 1.0pt" width="39">1</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 128.5pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="171">动物细胞核移植</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 100.75pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="134">卵(母)细胞</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 156.8pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1.5pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="209">细胞大，细胞质能有效调控核发育</td></tr><tr style="HEIGHT: 16.6pt; mso-yfti-irow: 2"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1.5pt solid; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 29.55pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-top-alt: solid windowtext 1.0pt" width="39">2</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 128.5pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="171">体细胞诱变育种</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 100.75pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="134">愈伤组织细胞</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 156.8pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1.5pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="209">分裂能力强。易诱发突变</td></tr><tr style="HEIGHT: 16.6pt; mso-yfti-irow: 3"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1.5pt solid; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 29.55pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-top-alt: solid windowtext 1.0pt" width="39">3</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 128.5pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="171">植物体细胞杂交培育新品种</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 100.75pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="134">花粉粒</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 156.8pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1.5pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="209">易于获取，易于培养</td></tr><tr style="HEIGHT: 16.6pt; mso-yfti-irow: 4; mso-yfti-lastrow: yes"><td style="BORDER-BOTTOM: windowtext 1.5pt solid; BORDER-LEFT: windowtext 1.5pt solid; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 29.55pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-top-alt: solid windowtext 1.0pt" width="39">4</td><td style="BORDER-BOTTOM: windowtext 1.5pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 128.5pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="171">烧伤患者皮肤细胞移植</td><td style="BORDER-BOTTOM: windowtext 1.5pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 100.75pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="134">自体皮肤生发层细胞</td><td style="BORDER-BOTTOM: windowtext 1.5pt solid; BORDER-LEFT: medium none; PADDING-BOTTOM: 0cm; PADDING-LEFT: 0cm; WIDTH: 156.8pt; PADDING-RIGHT: 0cm; HEIGHT: 16.6pt; BORDER-TOP: medium none; BORDER-RIGHT: windowtext 1.5pt solid; PADDING-TOP: 0cm; mso-border-left-alt: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt" width="209">分裂能力强，且不会引起免疫排斥</td></tr></tbody></table>

  A.1

  B.2

  C.3

  D.4

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• 4在动物细胞培养过程中，下列处理不恰当的是

  A.培养液和培养用具要进行无菌处理

  B.提供种类齐全的营养物质

  C.提供纯氧以保证细胞的呼吸作用

  D.保证提供适宜的温度和pH

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• 5下列有关植物细胞工程应用的叙述，错误的是

  A.利用组织培养技术培育脱毒苗，获得具有抗病毒的新品种

  B.利用组织培养技术获得人工种子，大多能保持亲本的优良性状

  C.利用细胞培养技术获得紫草素，实现了细胞产物的工厂化生产

  D.利用植物体细胞杂交技术获得“萝卜—甘蓝”，克服不同生物远缘杂交不亲和的障碍

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• 6下列关于植物组织培养和动物细胞培养的相关叙述中，正确的是

  A.动物细胞培养的原理是细胞增殖

  B.动物细胞培养过程也需要进行脱分化处理

  C.目前利用动物细胞培养技术已能产生新的动物个体

  D.植物组织培养要用一定的方法将细胞分散开来

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• 7在动物细胞核移植技术的应用过程中，用于核移植的供体细胞一般都选用10代以内的培养细胞，其主要原因是

  A.传代培养的细胞一般传至10代后不易传下去

  B.10代以内的细胞一般能保持保持正常的二倍体核型，保证供体细胞正常的遗传基础

  C.10代以后细胞的核型一定会发生变化，朝着癌细胞方向发展

  D.10代以内的细胞不能产生变异，可保持细胞正常的遗传基础

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