医药学论文：早期给予黄芪注射液治疗大鼠脑出血对血肿周围胞凋亡的影响

【摘要】 目的 探讨大鼠实验性脑出血后早期给予黄芪注射液对血肿周围细胞凋亡及细胞凋亡因子Caspase-3的影响。方法 将脑出血模型大鼠随机分成4个治疗组（手术同时治疗组、术后6h治疗组、术后12h治疗组、术后24h治疗组）和1个出血对照组。各治疗组每日给予腹腔注射黄芪注射液1次。然后各组分别于不同时期处死，取脑，连续切片作TUNEL染色和Caspase-3免疫组化染色。观察不同时间给药后对大鼠神经功能缺损、血肿周围细胞凋亡及凋亡相关蛋白Caspase-3指标的影响。结果 各治疗组血肿周围细胞凋亡率、Caspase-3蛋白表达均明显降低，各治疗组的神经功能评分较对照组明显改善，其中手术同时治疗组和术后6h治疗组与术后12h治疗组、术后24h治疗组比较上述各项观察指标差异有显著性（P< 0.01）。结论 早期应用黄芪注射液治疗大鼠脑出血能够显著降低血肿周围区域的细胞凋亡率和Caspase-3蛋白表达率。

 

【关键词】 脑出血；黄芪注射液；细胞凋亡；大鼠

 

The effect of astragalus propinquns injection on cell apoptosis around the hematoma in the early time after the experimental intracerebral hemorrhage in rats

 

【Abstract】 Objective To study the effect of astragalus propinquns injection on apoptotic cell and caspase-3 protein in the early time after the experimental intracerebral hemorrhage（ICH） in rats. Methods All the ICH rats about the experiment were randomly divided into the remedial group injecting astragalus propinquns and the group without injection. Then the remedial group of rats were divided into four groups which were the instantly treated group， the 6 hours treated group， the 12 hours treated group and the 24 hours treated group. The remedial groups were injected with astragalus propinquns injection once a day. After that all ICH rats were killed on different days to take out their brain in which the immunohistochemical method was performed by using the antibody of caspase-3 protein and TUNEL method was used to detect apoptosis around the hematoma in the brain. To observe the effect of astragalus propinquns injection in different time on the neurological dysfunction， caspse-3 protein expression and cell apoptosis around the hematoma of the brain.Results Compared with the hemorrhage group， the apoptotic rate and the positive cells of caspase-3 protein in the remedial groups were significantly decreased (P< 0.01) and neurological dysfunctions were significantly improved .The apoptotic rate of the instantly treated group and 6 hours later treated group decreased significantly compared with that of the 24 hours later treated group(P< 0.01).Conclusion When astragalus propinquns injection was used earlier to cure ICH， it can decrease the rate of cell apoptosis and caspase-3 protein expression around the hematoma significantly.

 

【Key words】 brain hemorrhage; astragalus propinquns injection;cell apoptosis;

 

脑出血是一种严重危害人类生命健康的临床常见病和多发病。据统计，在我国，脑出血占急性脑血管病（脑卒中）的40%～50%，是脑卒中死亡率最高的临床类型，脑出血后30天内病死率接近50%，许多存活者遗留明显的神经功能缺失［1］。目前对高血压脑出血的治疗方法虽较以往有很大进步，如超早期手术、微创技术清除血肿和综合治疗等［2］，但由于影响脑出血疗效的因素是多方面的，脑出血仍具有相当高的死亡率。近年来， 有人尝试采用活血化瘀药黄芪治疗急性期的脑出血，取得了一定的效果［3］，但目前对于该药治疗脑出血的时机和机制问题尚不清楚。基于此问题，本实验欲在不同时间给予黄芪注射液，采用TUNEL染色和Caspase-3免疫组化染色的方法，观察大鼠神经功能缺损、血肿周围细胞凋亡及凋亡相关蛋白Caspase-3指标，以期达到早期应用黄芪治疗脑出血后观察其疗效的目的。