医药学论文：苦参素对成纤维细胞增殖、形态学及转化生长因子β1的影响

［摘要］　目的：研究苦参素对成纤维细胞增殖、形态学及转化生长因子β1(TGF-β1)表达的影响，阐明其抗肝纤维化的作用机制。方法：用甲基噻唑基四唑MTT(methyl thiazolyl tetrazolium)比色法、HE染色及免疫细胞化学技术分别检测小鼠皮肤成纤维细胞(NIH3T3)增殖、形态学及TGF-β1的表达。结果：苦参素能明显抑制成纤维细胞增殖及TGF-β1的表达，并呈剂量依赖性。结论：苦参素可通过抑制成纤维细胞增殖及TGF-β1的表达而起到抗肝纤维化作用。

Affect of kwoninone on fibroblastic proliferation, morphology and expression of TGF-β1

 

　　［ABSTRACT］　AIM:To study the affect of kwoninone on fibroblastic proliferation, morphology and expression of transforming growth factor-beta (TGF-β1) and elucidate its mechanism in fibrosis.　METHODS: The proliferation, morphology and expression of TGF-β1 of fibroblast was measured by MTT bioassay, HE dyeing and immunocytochemistry.　RESULTS: The kwoninone has an could significantly inhibit fibroblastic proliferation, growth and expression of TGF-β1 in concentration-dependent manner.　CONCLUSION: The kwoninone anti-hepatic fibrosis effect through inhibiting fibroblastic proliferation, growth, and expression of TGF-β1.