5个月婴儿，双胎之小，肤色苍白3个月，呈加重趋势，食欲减退，平时易感冒，生后人工喂养，未加辅食。査体：发育营养差，皮肤黏膜苍白，心前区有1/6级收缩期杂音，肝肋下3cm,脾肋下1cm。血常规：Hb55g/L,RBC3.0x1012/L,白细胞、血小板及网织红细胞均正常。最有助于确立诊断的检查是

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