下列是生物研究中常用的实验方法，不正确的是-最需题库

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1用某种大小相似的绿色植物轮藻叶片分组进行光合作用实验：已知叶片实验前重量相等，在不同温度下分别暗处理1h，测其重量变化，立即再光照1h（光强度相同），再测其重量变化。得到如下结果，以下说法错误的是<table border="1" cellspacing="0" cellpadding="0" width="455" _dj="MsoNormalTable"><tbody><tr><td width="216">组别</td><td width="60">一</td><td width="60">二</td><td width="60">三</td><td width="60">四</td></tr><tr><td width="216">温度/℃</td><td width="60">27</td><td width="60">28</td><td width="60">29</td><td width="60">30</td></tr><tr><td width="216">暗处理后重量变化/mg</td><td width="60">－1</td><td width="60">－2</td><td width="60">－3</td><td width="60">－1</td></tr><tr><td width="216">光照后与暗处理前重量变化/mg</td><td width="60">+3</td><td width="60">+3</td><td width="60">+3</td><td width="60">+1</td></tr></tbody></table>
A.该轮藻呼吸作用酶的最适温度约为29℃
B.光照时，第一、二、三组轮藻释放的氧气量相等
C.光照时，第四组轮藻光合作用强度大于呼吸作用强度
D.光照时，第四组轮藻合成葡萄糖总量为3mg
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2某同学想探究二氧化碳浓度与光合作用速率的关系。他取A、B、C、D四株都有5张叶片的小白菜，用直径1cm的打孔器打取小叶圆片各10片，并设法抽去气体使之下沉，置于光下。取100mL三角瓶5个，编号1～4，按下表操作（光照、温度相同且适宜）并记录结果。下列评价或修正不合理的是<table style="BORDER-BOTTOM: medium none; BORDER-LEFT: medium none; MARGIN: auto 6.75pt; BORDER-COLLAPSE: collapse; BORDER-TOP: medium none; BORDER-RIGHT: medium none; mso-border-alt: solid windowtext .5pt; mso-table-lspace: 9.0pt; mso-table-rspace: 9.0pt; mso-table-anchor-vertical: paragraph; mso-table-anchor-horizontal: margin; mso-table-left: center; mso-table-top: 4.1pt; mso-padding-alt: 0cm 5.4pt 0cm 5.4pt; mso-border-insideh: .5pt solid windowtext; mso-border-insidev: .5pt solid windowtext" border="1" cellspacing="0" cellpadding="0" align="left" _dj="MsoNormalTable"><tbody><tr style="PAGE-BREAK-INSIDE: avoid; mso-yfti-irow: 0; mso-yfti-firstrow: yes"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1pt solid; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 26.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt" rowspan="2" width="35">编号</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 293.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" width="391" colspan="4">实验处理</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 79.2pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1pt solid; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt" valign="top" rowspan="2" width="106">30min内上浮叶圆片数（片）</td></tr><tr style="PAGE-BREAK-INSIDE: avoid; mso-yfti-irow: 1"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">叶圆片来源</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 81pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="108">叶圆片数（片）</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">自来水（mL）</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 68.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="91">NaHCO3(克)</td></tr><tr style="PAGE-BREAK-INSIDE: avoid; mso-yfti-irow: 2"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1pt solid; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 26.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="35">1</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">A</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 81pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="108">10</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">40</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 68.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="91">0</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 79.2pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="106">2</td></tr><tr style="PAGE-BREAK-INSIDE: avoid; mso-yfti-irow: 3"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1pt solid; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 26.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="35">2</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">B</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 81pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="108">10</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">40</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 68.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="91">1</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 79.2pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="106">6</td></tr><tr style="PAGE-BREAK-INSIDE: avoid; mso-yfti-irow: 4"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1pt solid; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 26.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="35">3</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">C</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 81pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="108">10</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">40</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 68.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="91">3</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 79.2pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="106">4</td></tr><tr style="PAGE-BREAK-INSIDE: avoid; mso-yfti-irow: 5; mso-yfti-lastrow: yes"><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: windowtext 1pt solid; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 26.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="35">4</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">D</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 81pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="108">10</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 72pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="96">40</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 68.55pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="91">5</td><td style="BORDER-BOTTOM: windowtext 1pt solid; BORDER-LEFT: #000000; PADDING-BOTTOM: 0cm; BACKGROUND-COLOR: transparent; PADDING-LEFT: 5.4pt; WIDTH: 79.2pt; PADDING-RIGHT: 5.4pt; BORDER-TOP: #000000; BORDER-RIGHT: windowtext 1pt solid; PADDING-TOP: 0cm; mso-border-alt: solid windowtext .5pt; mso-border-left-alt: solid windowtext .5pt; mso-border-top-alt: solid windowtext .5pt" valign="top" width="106">5</td></tr></tbody></table>
A.自变量二氧化碳浓度的控制不严格
B.制备的叶圆片在投入三角瓶之前应放于黑暗处
C.实验材料本身存在的差异会影响实验结果
D.只要控制光照、温度相同即可
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3在生物体的下列生理活动过程中，没有ADP生成的是
A.浆细胞分泌抗体
B.胃蛋白酶对蛋白质的分解
C.叶绿体内C5的生成
D.番茄根细胞对Mg2+的吸收
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4下列有关ATP的叙述，正确的是
A.线粒体是蓝藻细胞产生ATP的主要场所
B.叶绿体中消耗ATP的场所是类囊体薄膜
C.ATP分子由1个腺嘌呤和3个磷酸基团组成
D.细胞连续分裂时，伴随着ATP与ADP的相互转化
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5绿色植物的叶肉细胞内，合成糖类和消耗糖类的结构分别是
A.叶绿体基质、线粒体基质
B.核糖体、染色体
C.高尔基体、内质网
D.叶绿体基质、细胞质基质
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6下面是四位同学的实验操作方法或结果，其中错误的一项是
A.番茄汁中含有丰富的葡萄糖和果糖，可用作还原糖鉴定的材料
B.纸层析法分离叶绿体中的色素，其颜色从上到下依次是：橙黄色、黄色、蓝绿色和黄绿色
C.蛋白质与双缩脲试剂作用产生紫色反应
D.显微镜下观察根尖的有丝分裂，在细胞呈正方形的区域易找到分裂期的细胞
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7下列关于细胞生命历程的叙述不正确的是
A.正常的细胞会随着分裂次数的增加而衰老
B.细胞分化是有关基因发生改变造成的
C.低温引起的细胞冻伤和死亡属于细胞坏死
D.受遗传机制决定的细胞程序性死亡属于细胞凋亡
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8关于人体癌细胞的叙述，不正确的是
A.癌变主要是因为人体细胞的原癌基因和抑癌基因发生突变
B.癌细胞比所有正常细胞分裂周期长、生长快
C.有些病毒能将其基因整合到人的基因组中，从而诱发人的细胞癌变
D.癌细胞的形态结构和表面的物质都发生明显的变化
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9假设取一个含有一对同源染色体的受精卵，用32P标记细胞核中的DNA，然后放在不含32P的培养基中培养，让其连续进行两次有丝分裂，形成四个细胞，这四个细胞中含32P的细胞个数可能是
A.2
B.3
C.4
D.前三项都有可能
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